1. Select human or mouse
- Choose the species from which the proteins were obtained because a different model was developed for each species.
- Prediction for proteins from other species is technically possible, but the results should be used with extra caution.
2. Provide protein sequences in FASTA format
- Paste a single sequence or upload a FASTA file containing multiple protein sequences.
- Protein sequences can be up to 8,000 amino acids.
- Each sequence must include a FASTA header.
Download examples for an individual human protein, an individual mouse protein, multiple human proteins, or multiple mouse proteins.
3. Submit for prediction
- An individual protein usually takes several seconds to process.
- A FASTA file containing multiple protein sequences may take longer.
- Prediction runs locally in the browser; keep the page open until it completes.
4. Interpret and export results
- A prediction score is reported for every serine or threonine residue.
- Scores greater than 0.5, 0.95, and 0.99 can be regarded as low, medium, and high confidence, respectively.
- Filter the result table, copy visible rows, or download the complete filtered result as CSV.