Tutorial

Please select species: 'human' or 'mouse'

• For better results, it is recommended to choose appropriate species from which the proteins are, since different models were developed for each species.
• Prediction for proteins from other species is technically possible, but the results should be used with extra caution!

Paste the protein sequence(s) in fasta format

• Either a single sequence (example for human / example for mouse) or a file containing multiple proteins sequences (example for human / example for mouse) is acceptable.
• There is no limit in the length of a protein sequence (can be up to 8000 A.A.)
• Besides the protein sequence, the header must be included into the fasta file.

Click the 'submit for prediction' button for prediction

• For individual proteins, it usually takes several seconds to get the results.
• For a fasta file containing multiple protein sequences, it may take longer.

View the prediction results

• A prediction score is given for each S/T residue.
• Sites with scores >0.5, >0.95, > 0.99 can be regarded as low confidence, medium confidence, and high confidence, respectively.
• Results can be downloaded for further analysis if needed.