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Using the interaction database

OGT-PIN tutorial

Learn how to search OGT interaction records and interpret their curated evidence fields.

What is OGT-PIN?

OGT Protein Interaction Network (OGT-PIN) is a comprehensive database of experimentally identified interacting proteins of OGT and its orthologues.

Terms used in OGT-PIN

  • Unique identifier for interactor A. UniProt accession of OGT or an orthologue, such as O15294 for human OGT.

  • Gene name for interactor A. Gene symbol of OGT or an orthologue, such as SXC or SEC.

  • NCBI Taxonomy identifier for interactor B. Species used in the original study, including Homo sapiens, Mus musculus, Rattus norvegicus, Drosophila melanogaster, Arabidopsis thaliana, and others.

  • Unique identifier for interactor B. UniProt accession of the interacting protein, such as O60502.

  • Gene name for interactor B. Gene symbol of the interacting protein, such as OGA.

  • Protein name for interactor B. Primary protein name of the interacting protein, such as protein O-GlcNAcase.

  • Interaction detection method. Method used to determine the interacting protein.

  • PMID. PubMed identifier of the original publication that identified the interacting protein.

  • Analytical throughput. Low throughput or high throughput.

  • Source. Source that compiled the interaction. “GUMC” denotes data curated by the GUMC team after surveying O-GlcNAc-focused studies.

  • Curator comments. Curator notes for specific interactions. “Caution” indicates that an entry’s validity may be limited based on the method description in the original study and the IMEx consortium curation manual (version 01/05/2015).

  • Meeting IMEx criteria. Proteins meeting the IMEx curation manual recommendations are labeled “Yes.”

  • High-stringency interactor. Proteins meeting more stringent rules are labeled “Yes”; others are labeled “No.”

How do I use OGT-PIN?

  1. Obtain a UniProt accession or gene symbol for the protein of interest.
  2. Choose the corresponding OGT-PIN search field and submit the identifier.
  3. Filter the result table to narrow records by details such as species.
  4. Copy the visible rows or download the complete filtered result as CSV.

Search tips

  • Check spelling carefully because there is no spell checker.
  • Searches are not case-sensitive; OGA and oga are equivalent.