O-GlcNAc Database

Details for Accession #: Q7Z6Z7

Protein Sequence

>sp|Q7Z6Z7|HUWE1_HUMAN E3 ubiquitin-protein ligase HUWE1 OS=Homo sapiens OX=9606 GN=HUWE1 PE=1 SV=3
MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDR
FDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLT
TLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLA
ECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIM
ESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSI
LYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLP
VLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLG
DEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQ
RPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSL
KHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREV
LGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLG
SAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNH
AAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESI
LSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKV
LQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAH
AYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSL
PSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLA
PMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVR
QRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSP
MLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLD
AWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRK
PLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQ
QLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIA
MSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFT
DTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAAL
PLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKL
LEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWF
DDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVML
TLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEET
KIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKS
TRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSG
SLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPN
AVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLL
QDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESR
DGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSE
LIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAAL
GRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKG
LVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQ
GASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQ
EMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANR
EDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDD
DGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMV
RHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRL
LGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGT
LSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEE
ETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKE
TLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAET
TQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSS
EQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADS
RGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPS
TNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVT
FIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSA
LSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLL
LDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCI
ETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNI
FQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETN
CESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGE
TSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGA
SSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTA
TTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLED
AANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCET
LSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQ
KFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVR
EGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEER
PPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPV
RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAE
THRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVH
VRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYAL
FRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKS
VRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTE
ENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDID
DLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGI
QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Peptide Information

ID	Peptide Seq	Site Residue	Position in Peptide	Position in Protein
4111	LFGHSSTSALSAILR	S		3122.0
4112	QLDSILSSL	S		853.0
4113	QLDSILSSL	S		854.0
4114	QMGGSSSHNRPSGSNVDTLLRL	S		3159.0
4115	QMGGSSSHNRPSGSNVDTLLRL	S		3160.0
5522	DSAVAISGADSR	S	7	2936.0
5526	DQSAQCTASK	T	7	2722.0
10006196	RQRQLMHERLFGHSSTSALSAILRSPAFTSR	T		3118
10006197	ARQRQLMHERLFGHSSTSALSAILRSPAFTS	S		3117
10008831	DSAVAISGADSR	S	7	2936
10009307	QQQAATSESSQSEASVR	S	7	3794
10009370	SDGSGESAQPPEDSSPPASSESSSTR	S	19	2922
10009371	SDGSGESAQPPEDSSPPASSESSSTR	S	20	2923
10016886	LFGHSSTSALSAILR	S	6	3117
10017576	SDGSGESAQPPEDSSPPASSESSSTR	S	19	2922
10017577	SDGSGESAQPPEDSSPPASSESSSTR	S	19	2922
10017578	SDGSGESAQPPEDSSPPASSESSSTR	S	20	2923
10019791				3116
10019879				3118
10024448				3118
10026225	AQCETLSPDGLPEEQPQTTK	S	7	3662
10028190	LFGHSSTSALSAILR	S	5	3116
10028965	LFGHSSTSALSAILR	T	7	3118
10029608	LFGHSSTSALSAILR	S	5	3116
10029609	LFGHSSTSALSAILR	T	7	3118
10031904		T		3118
10034867	SSESELCIETPK	T	10	3242
10035209	QQQAATSESSQSEASVR	S	7	3794

O-GlcNAcylation Information

ID	Method	Analytical Throughput	Ambiguity	Species	Sample Type	Data Source PMID	Comments
4111	MS	HTP	None	human	T cells	29351928	None
4112	MS	HTP	None	human	T cells	29351928	None
4113	MS	HTP	None	human	T cells	29351928	None
4114	MS	HTP	None	human	T cells	29351928	None
4115	MS	HTP	None	human	T cells	29351928	None
5522	MS	HTP	None	human	HeLa cells	31373491	None
5526	MS	HTP	None	human	HeLa cells	31373491	None
10006196	MS	HTP	None	human	HeLa cells	30059200	None
10006197	MS	HTP	None	human	HeLa cells	30059200	None
10008831	MS	HTP	None	human	HepG2 cells	30620550	None
10009307	MS	HTP	None	human	HepG2 cells	30620550	None
10009370	MS	HTP	None	human	HEK 293T cells	30620550	None
10009371	MS	HTP	None	human	HEK 293T cells	30620550	None
10016886	MS	HTP	None	human	HeLa cells	30059200	None
10017576	MS	HTP	None	human	HepG2 cells	30620550	None
10017577	MS	HTP	None	human	HEK 293T cells	30620550	None
10017578	MS	HTP	None	human	HEK 293T cells	30620550	None
10019791	MS	HTP	None	human	HeLa cells	34161081	None
10019879	MS	HTP	None	human	HeLa cells	34161081	None
10024448	MS	HTP	None	human	colorectal cancer (CRC) cell lines	35254053	None
10026225	MS	HTP	None	human	Jurkat cells, MCF-7 cells, K562 cells	35132862	None
10028190	MS	HTP	None	human	HeLa cells	35254053	None
10028965	MS	HTP	None	human	HeLa cells	35254053	None
10029608	MS	HTP	None	human	SW480 cells	35254053	None
10029609	MS	HTP	None	human	SW480 cells	35254053	None
10031904	MS	HTP	None	human	SW480 cells, SW620 cells	35254053	None
10034867	MS	HTP	None	human	Jurkat cells	35705054	None
10035209	MS	HTP	None	human	Jurkat cells	35705054	None

ID	Peptide Seq	Site Residue	Position in Peptide	Position in Protein	Method	Analytical Throughput	Ambiguity	Species	Sample Type	Data Source PMID	Comments
4111	LFGHSSTSALSAILR	S		3122.0	MS	HTP	None	human	T cells	29351928	None
4112	QLDSILSSL	S		853.0	MS	HTP	None	human	T cells	29351928	None
4113	QLDSILSSL	S		854.0	MS	HTP	None	human	T cells	29351928	None
4114	QMGGSSSHNRPSGSNVDTLLRL	S		3159.0	MS	HTP	None	human	T cells	29351928	None
4115	QMGGSSSHNRPSGSNVDTLLRL	S		3160.0	MS	HTP	None	human	T cells	29351928	None
5522	DSAVAISGADSR	S	7	2936.0	MS	HTP	None	human	HeLa cells	31373491	None
5526	DQSAQCTASK	T	7	2722.0	MS	HTP	None	human	HeLa cells	31373491	None
10006196	RQRQLMHERLFGHSSTSALSAILRSPAFTSR	T		3118	MS	HTP	None	human	HeLa cells	30059200	None
10006197	ARQRQLMHERLFGHSSTSALSAILRSPAFTS	S		3117	MS	HTP	None	human	HeLa cells	30059200	None
10008831	DSAVAISGADSR	S	7	2936	MS	HTP	None	human	HepG2 cells	30620550	None
10009307	QQQAATSESSQSEASVR	S	7	3794	MS	HTP	None	human	HepG2 cells	30620550	None
10009370	SDGSGESAQPPEDSSPPASSESSSTR	S	19	2922	MS	HTP	None	human	HEK 293T cells	30620550	None
10009371	SDGSGESAQPPEDSSPPASSESSSTR	S	20	2923	MS	HTP	None	human	HEK 293T cells	30620550	None
10016886	LFGHSSTSALSAILR	S	6	3117	MS	HTP	None	human	HeLa cells	30059200	None
10017576	SDGSGESAQPPEDSSPPASSESSSTR	S	19	2922	MS	HTP	None	human	HepG2 cells	30620550	None
10017577	SDGSGESAQPPEDSSPPASSESSSTR	S	19	2922	MS	HTP	None	human	HEK 293T cells	30620550	None
10017578	SDGSGESAQPPEDSSPPASSESSSTR	S	20	2923	MS	HTP	None	human	HEK 293T cells	30620550	None
10019791				3116	MS	HTP	None	human	HeLa cells	34161081	None
10019879				3118	MS	HTP	None	human	HeLa cells	34161081	None
10024448				3118	MS	HTP	None	human	colorectal cancer (CRC) cell lines	35254053	None
10026225	AQCETLSPDGLPEEQPQTTK	S	7	3662	MS	HTP	None	human	Jurkat cells, MCF-7 cells, K562 cells	35132862	None
10028190	LFGHSSTSALSAILR	S	5	3116	MS	HTP	None	human	HeLa cells	35254053	None
10028965	LFGHSSTSALSAILR	T	7	3118	MS	HTP	None	human	HeLa cells	35254053	None
10029608	LFGHSSTSALSAILR	S	5	3116	MS	HTP	None	human	SW480 cells	35254053	None
10029609	LFGHSSTSALSAILR	T	7	3118	MS	HTP	None	human	SW480 cells	35254053	None
10031904		T		3118	MS	HTP	None	human	SW480 cells, SW620 cells	35254053	None
10034867	SSESELCIETPK	T	10	3242	MS	HTP	None	human	Jurkat cells	35705054	None
10035209	QQQAATSESSQSEASVR	S	7	3794	MS	HTP	None	human	Jurkat cells	35705054	None